AGRO PATH FINDER • SKNAU JOBNER 2025
Techniques in Plant Biotechnology
B.Sc. (Hons.) Agriculture • Semester 7 • BIOTECH 411
Lecture-wise study materialsTheory & PracticalPDF • MCQs • Videos
Imported outline — verification pending
This schedule comes from the supplied HTML pack. Check the original university syllabus before treating wording, numbering or topic coverage as final. Numbering gaps in the supplied outline have been flagged for review; missing topics have not been invented.
Theory Lecture Schedule
19 indexed topics01
Concepts of Plant Biotechnology: History of Plant Tissue Culture and] Plant Genetic Engineering, Scope and importance in Crop Improvement
02
Totipotency and Morphogenesis; Nutritional requirements of in-vitro cultures
03
Techniques of In-vitro cultures- Anther culture, Pollen culture, Ovule culture, Embryo culture, Test tube fertilization, Endosperm culture, Factors affecting above in-vitro cultures. Applications and Achievements
04
Micropropagation
05
Protoplast isolation, culture and regeneration; Somatic hybridization] (somatic hybrids and cybrids) and its application in crop improvement
06
Somatic embryogenesis
07
Micropropagation technique for the generation of quality planting] material
09
Protoplast isolation, Culture, and Fusion; Products of somatic hybrids and cybrids, Applications in crop improvement.
10
Synthetic seed production technology
11
Genetic engineering; Restriction enzymes
12
Direct and indirect method of gene transfer
13
Vectors for gene transfer
14
Gene cloning
15
Transgenic plants and their applications
16
Blotting techniques DNA finger printing, ,.
17
DNA finger printing
18
DNA based markers: RFLP, AFLP, RAPD, SSR and DNA Probes
19
Mapping QTL: Future prospects
20
MAS, and its application in crop improvement Total
Practical Lecture Schedule
13 indexed topics01
Requirements for Plant Tissue Culture Laboratory
02
Techniques in Plant Tissue Culture
03
Media components and preparations
04
Sterilization techniques and Inoculation of various explants
05
Aseptic manipulation of various explants
06
Callus induction and Plant Regeneration
07
Micro propagation of important crops
08
Anther, Embryo and Endosperm culture
09
Hardening / Acclimatization of regenerated plants T
10
Somatic embryogenesis and synthetic seed production
11
Demonstration of Isolation and Culturing of protoplast
12
Demonstration of Isolation of DNA
13
Demonstration of gel-electrophoresis techniques & PCR T
This page indexes the supplied topic outline. Lecture notes, PDFs, videos and tests will be linked only when those resources are available.
