AGRO PATH FINDER • SKNAU JOBNER 2025

Biotechnology of Crop Improvement

B.Sc. (Hons.) Agriculture • Semester 7 • BIOTECH 412

Elective in supplied directoryLecture-wise study materialsTheory & PracticalPDF • MCQs • Videos
Imported outline — verification pending

This schedule comes from the supplied HTML pack. Check the original university syllabus before treating wording, numbering or topic coverage as final. Numbering gaps in the supplied outline have been flagged for review; missing topics have not been invented.

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Semester 7 elective: This subject is listed under elective course options in the source syllabus.

Theory Lecture Schedule

10 indexed topics
01
Impact of Biotechnology on crop improvement and the perspective of society
Theory lecture • Source page reference in supplied pack: 242
Notes coming soon
02
Various biotechnological techniques available for crop improvement — Plant Tissue Culture, Genetic Engineering, Marker Assisted breeding and Genomic Selection
Theory lecture • Source page reference in supplied pack: 242
Notes coming soon
03
Biosafety regulations and their application in Agricultural Biotechnology
Theory lecture • Source page reference in supplied pack: 242
Notes coming soon
04
Somacional variation and its use in crop improvement
Theory lecture • Source page reference in supplied pack: 243
Notes coming soon
05
Techniques of protoplast culture, regeneration and somatic cell hybridization, achievements and limitations, utility in the improvement of crop plants
Theory lecture • Source page reference in supplied pack: 243
Notes coming soon
06
Embryo cuiture; anther/pollen culture; artificial seeds
Theory lecture • Source page reference in supplied pack: 243
Notes coming soon
07
Direct and Indirect methods of gene transfer in plants – Agrobacterium-mediated gene transfer in dicots and monocots: Direct DNA delivery methods (microinjection, particle gun method, electroporation); gene targeting
Theory lecture • Source page reference in supplied pack: 243
Notes coming soon
09
Gene silencing techniques; introduction to siRNA; siRNA technology; Micro RNA; construction of siRNA vectors; principle and application of gene silencing
Theory lecture • Source page reference in supplied pack: 243
Notes coming soon
10
Creation of transgenic plants; debate over GM crops
Theory lecture • Source page reference in supplied pack: 243
Notes coming soon
11
Genome editing-concept, strategies and applications
Theory lecture • Source page reference in supplied pack: 243
Notes coming soon

Practical Lecture Schedule

6 indexed topics
01
Agrobacterium-mediated transformation in Tobacco — preparation of construct, transfer to binary vector
Practical lecture • Source page reference in supplied pack: 243
Notes coming soon
02
Transform Agrobacterium, prepare explant, Inoculation and Co- cultivation
Practical lecture • Source page reference in supplied pack: 243
Notes coming soon
03
Antibiotic based selection of putative transformants, validation using PCR
Practical lecture • Source page reference in supplied pack: 243
Notes coming soon
04
Genome editing- preparation of CRISPR/CAS construct
Practical lecture • Source page reference in supplied pack: 243
Notes coming soon
05
Direct transfer to plant, analysis of the targets
Practical lecture • Source page reference in supplied pack: 243
Notes coming soon
06
Planning of a MABB programme — selection of parents, crossing strategies, marker analysis
Practical lecture • Source page reference in supplied pack: 243
Notes coming soon
This page indexes the supplied topic outline. Lecture notes, PDFs, videos and tests will be linked only when those resources are available.