AGRO PATH FINDER • SKNAU JOBNER 2025
Fundamentals of Agri Biotechnology
B.Sc. (Hons.) Agriculture • Semester 6 • BIOTECH 321
Lecture-wise study materialsTheory & PracticalPDF • MCQs • Videos
Imported outline — verification pending
This schedule comes from the supplied HTML pack. Check the original university syllabus before treating wording, numbering or topic coverage as final. Numbering gaps in the supplied outline have been flagged for review; missing topics have not been invented.
Theory Lecture Schedule
21 indexed topics01
Introduction to Plant Tissue Culture and Genetic Engineering: History; Cellular totipotency and cytodifferentiation
02
Callus culture, Single-cell/suspension culture and their) applications; Organogenesis and somatic embryogenesis
03
Somaclonal variation and its use in crop improvement
04
Embryo rescue technique and its significance in hybrid; development; In vitro fertilization, ovule culture and_ its significance in hybrid development
05
Protoplast isolation, culture and _—_ regeneration; + Somatic hybridization (somatic hybrids and cybrids) and its application in crop improvement
06
Anther and pollen culture for haploid production; Development of disease-free (virus free) plants through apical meristem culture
07
Micropropagation technique for the generation of quality planting material
09
Synthetic seeds and its applications
10
National certification and Quality management of TC plants
11
Secondary metabolite production, in-vitro germplasm conservation
12
Introduction to Molecular Biology: DNA structure, structure and function; RNA, types and function
13
Central Dogma- DNA replication, transcription and translation
14
Structure of prokaryotic and eukaryotic gene; Genetic code
15
Lac Operon concept
16
Nucleic acid hybridization; Polymerase chain reactionand its applications
17
DNA sequencing — Sanger method
18
Introduction to recombinant DNA technology: DNA modifying enzymes and vectors
19
Plant genetic transformation — physical (Gene gun method), chemical (PEG mediated) and Agrobacterium-mediated gene transfer methods
20
Importance of transgenics and genome editing
21
‘ Biosafety
22
Introduction to various molecular markers: RFLP, RAPD, SSR, SNP etc.; Marker-assisted breeding in crop improvement
Practical Lecture Schedule
8 indexed topics01
Introduction to Plant Tissue Culture Laboratory
02
Good Laboratory Practices T
03
Glassware sterilization T
04
Media Preparation and sterilization 3 Micropropagation 4
06
Restriction digestion of plasmid DNA and agarose gel[ electrophoresis
07
Isolation of Plant genomic DNA and Quantification of DNA
08
PCR amplification of DNA and Gel electrophoresis of amplified T
09
Visit to tissue culture units /biotech labs T
This page indexes the supplied topic outline. Lecture notes, PDFs, videos and tests will be linked only when those resources are available.
